Figure 5.

Osmostress mediates a G1 cell-cycle delay in a p38 SAPK- and p57-dependent manner. (A) Wild-type, p38^−/− and p57^−/− MEFs were stressed with 100 mM NaCl. One hour later, nocodazole was added to trap the cells at the G2/M transition. Cell-cycle progression was monitored by FACS by collecting samples every 2 h. Representative DNA profiles are shown. (B) The percentage of wild-type, p38^−/− and p57^−/− MEFs in G₁ from three independent experiments is shown. Solid circles represent osmostressed MEF cells. Open circles are non-stressed control MEF cells.