Figure 3.

Inheritance of IBM1 intronic methylation and IBM1-L transcript accumulation. (A, C) DNA methylation of the IBM1 large intron was assayed in the indicated genotypes by Col-0-specific PCR from bisulphite-treated DNA that was followed by digestion with TaqI (A) or HpyCH4V (C). CG and CHG methylation protect the TaqI and HpyCH4V sites, respectively, from bisulphite conversion, which facilitates restriction digestion after bisulphite treatment and PCR. Undigested (U) and digested (D) samples are shown. (B, D) Allele-specific RT–PCR analysis of IBM1-L transcript accumulation in Ler-0 × met1 (B) Ler-0 × cmt3 and Ler-0 × kyp (D) F1 individuals. The Ler × Col-0 individuals are shown as controls. The ACTIN 2 (ACT2) gene was used as a control.