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. 2012 May 11;31(13):2981–2993. doi: 10.1038/emboj.2012.141

Figure 7.

Figure 7

IBM1 targets DNA demethylase genes in the met1 background. (A) CHG methylation in the gene body of ROS1 and a ROS1-target gene (AT1G26400) was determined in the WT, met1-3 and a met1::IBM1-L line (#23) using bisulphite sequencing. The full DNA methylation profiles are provided in Supplementary Figure S4. (B) Association of ROS1 and AT1G26400 with H3K9me2 was determined by ChIP and is represented as a relative enrichment over the WT. Quantifications are from three independent experiments (±standard error of the mean). (C) RT–PCR analysis of the ROS1, DME and AT1G26400 transcript accumulation in the indicated genotypes. The amplification of ACTIN 2 (ACT2) was used to normalize the RNA template levels. The negative controls (no RT) lacked reverse transcriptase.