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Sensitivity and specificity of the ALK FISH assay based on different number of tumor areas/nuclei scored compared with definitive status set by analysis of entire specimen following a contiguous sliding field strategy
| Number of tumor areas |
||||||
|---|---|---|---|---|---|---|
| 2 | 3 | 4 | 5 | 6 | 7 | |
| % Sensitivity | 98.6 | 99.3 | 100 | 100 | 100 | 100 |
| % Specificity | 96.6 | 100 | 100 | 100 | 100 | 100 |
| No. nuclei scored | ~30 | ~45 | ~60 | ~75 | ~90 | ~105 |
