Figure 5.

Lactobacillus-C. albicans cocultures for transcriptome analysis. The experimental setup employing a 0.45 μm membrane for separation of the bacterial and fungal cultures in 6-well tissue culture plates is represented schematically in (a). Results of a typical Lactobacillus-C. albicans coculture and C. albicans-only control experiment are shown in (b). Growth of C. albicans and Lactobacillus (Lb) in the respective compartments was determined by OD_600 nm readings (left y-axis). The pH of the culture media is depicted in dashed lines using the right y-axis. For transcriptome analysis, total RNA was isolated from C. albicans cells harvested from cocultures and controls.