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. Author manuscript; available in PMC: 2012 Jul 12.
Published in final edited form as: J Immunol. 2009 Apr 1;182(7):4226–4236. doi: 10.4049/jimmunol.0800771

Figure 1. B and T cell proliferation in lipogranulomas.

Figure 1

A, Immunohistochemistry of a TMPD-induced lipogranuloma (serial sections) demonstrating the presence of contiguous B cell (B220+) and T cell (CD3+) zones as well as cellular proliferation, as demonstrated by Ki-67 staining. Bottom right panel shows the absence of cells staining with the follicular dendritic cell marker FDC-M1 (FDC). B, Flow cytometry of lipogranuloma cells. Gates were set on either the B cells anti-CD45R (B220) or T cells (anti-CD4) and the percentage of cells staining with anti-Ki67 antibodies was determined. C, In vivo BrdU labeling of T and B cells in the lipogranulomas and spleens of TMPD-treated mice. Single cell suspensions were stained with anti-CD45R (B220), anti-CD3, and anti-BrdU antibodies. Data are expressed as the % of BrdU+ B cells or T cells, respectively.

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