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. Author manuscript; available in PMC: 2012 Jul 12.
Published in final edited form as: J Immunol. 2009 Apr 1;182(7):4226–4236. doi: 10.4049/jimmunol.0800771

Figure 5. IgG1 and IgG2a induced hypergammaglobulinemia in TMPD-treated mice is T cell dependent.

Figure 5

A, Serum samples were obtained from wild type C57BL/6J (WT, n = 5) or B6.129P2-Tcrbtm1MomTcrdTm1Mom (n = 6) mice treated 3 months earlier with TMPD. IgG1, IgG2a, IgG3, and IgM levels were measured by ELISA and means were compared by the Mann-Whitney test. B, IgG production in lipogranulomas. Lipogranuloma cells and splenocytes from two mice were tested in quadruplicate for T cell dependent immunoglobulin secretion (IgG1+IgG2a+IgG2b) by ELISPOT assay. C, Quantification of plasmablasts in spleen and lipogranulomas. Pooled lipogranuloma and spleen cells from four TMPD-treated BALBc/J mice were stained with anti-B220 and anti-CD138 antibodies and analyzed by flow cytometry.

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