Table 1. Antibodies Used Flow Cytometric Analysis.
| Antigen | Clone | Fluorochrome | Manufacturer |
|---|---|---|---|
| Akt (pS473) | M89-61 | PE | BD Biosciences |
| CD3 | 145-2C11 | APC-Cy7 | BD Biosciences |
| CD11b | M1/70 | PE-Texas Red | Invitrogen |
| CD11c | HL3 | APC | BD Biosciences |
| PE-Cy7 | BD Biosciences | ||
| CD19 | 1D3 | PE-Cy7 | BD Biosciences |
| Alexa Fluor 700 | BD Biosciences | ||
| CD40 | HM40-3 | Alexa Fluor 647 | BioLegend |
| CD69 | H1.2F3 | PerCP-Cy5.5 | BD Biosciences |
| CD80 | 16-10A1 | Pacific Blue | BD Biosciences |
| CD86 | GL1 | Alexa Fluor 700 | BD Biosciences |
| F4/80 | BM8 | eFluor 450 | eBioscience |
| Ly6G | 1A8 | PerCP-Cy5.5 | BD Biosciences |
| Ly6C | AL-21 | APC-Cy7 | BD Biosciences |
| MHC II (I-A/I-E) | M5/114.15.2 | FITC | eBioscience |
| APC-eFluor 780 | eBioscience | ||
| NF-κB (p65) | K10-985 | PECy7 | BD Biosciences |
| NK1.1 | PK136 | Alexa Fluor 700 | BD Biosciences |
| p38 | 36/p38 | APC | BD Biosciences |
| Siglec F | E50-2440 | PE | BD Biosciences |
| TLR2 | 6C2 | FITC | eBioscience |
| TLR4 | MTS510 | PE-Cy7 | eBioscience |
APC, Allophycocyanin. BioLegend, San Diego, CA, USA; eBioscience, San Diego, CA, USA.