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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Exp Cell Res. 2012 May 28;318(14):1745–1758. doi: 10.1016/j.yexcr.2012.04.020

Figure 1. Expression of IRS proteins in 32D cells.

Figure 1

A. 32D cells and cells transfected with eithera IRS1 or IRS2 cDNA were treated in the presence or absence of IL-3 (5% WEHI-3B supernatant) or IL-4 (10 ng/ml) for 30 minutes. Cell lysates were prepared and the western blot was probed with anti IRS1, anti IRS2, or anti GAPDH antibodies. B. Cells were treated in the presence or absence of IL-3 (5% WEHI-3B supernatant) or IL-4 (10 ng/ml) for 30 minutes. Cell lysates were prepared and immunoprecipitated with antibodies specific for IRS1, IRS2, or an isotype control antibody (IgG). The western blot was probed with an anti phosphotyrosine (pY) antibody.