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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Exp Cell Res. 2012 May 28;318(14):1745–1758. doi: 10.1016/j.yexcr.2012.04.020

Figure 2. IRS1 sensitizes 32D cells to vincristine induced cell death.

Figure 2

A. 32D cells were treated with various concentrations of vincristine, a microtubule assembly inhibitor, in the presence of IL-3, supplied as 5% WEHI-3B supernatant which contains 0.75% serum, for 24 hrs. The percentage of cells with <2N DNA content (hypodiploid) was measured by propidium iodide staining followed by FACs analysis. Representative histograms are shown. The percentage of sub diploid cells is indicated. B. 32D cells lacking or expressing IRS1 (clone 484.3) or IRS2 were treated with various concentrations of vincristine in the presence of IL-3, supplied as 5% WEHI-3B supernatant which contains 0.75% serum. The percentage of hypodiploid cells was measured by propidium iodide staining of nuclear DNA content followed by FACS analysis. The percentage of sub-diploid cells is indicated.