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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Exp Cell Res. 2012 May 28;318(14):1745–1758. doi: 10.1016/j.yexcr.2012.04.020

Figure 7. Decreasing Annexin A2 reduces 32D-IRS1 cell sensitivity to chemotherapy.

Figure 7

A. 32D-IRS1 (260.3) cells were infected with Annexin A2 shRNA and two cell lines were generated (101-2W3 and 101-2W4). Cell lysates were prepared and the western blot was probed with anti-Annexin A2, anti-IRS1 or anti-Tubulin. The images for each cell type were derived from the same exposure of the same film. Densitometry was used to quantify bands. B. 32D cells and transfectants were treated with 4 g/ml etoposide, in the presence of IL-3, supplied as 5% WEHI-3B supernatant which contains 0.75% serum, for 24 hours. The percentage of hypodiploid cells was measured by propidium iodide staining of nuclear DNA content followed by FACS analysis. The percentage of sub-diploid cells is shown. Two-tailed, unpaired, t-test was used to determine statistical significance. C. 32D cells and transfectants were pretreated in the presence or absence of 10 μM Q-VD-OPh for 1 hr and then treated in the presence or absence of 4 g/ml etoposide, for 20 hrs. Cells were analyzed by FACS to determine the percentage of PI positive cells. The percentage of PI positive cells was expressed as fold change relative to media control. Two-tailed, unpaired, t-test was used to determine statistical significance.