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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Stroke. 2011 Sep 22;42(12):3574–3579. doi: 10.1161/STROKEAHA.111.626648

Figure 4.

Figure 4

IL-6R up-regulates transcriptional activity of Mn-SOD via STAT3 recruitment into the Mn-SOD promoter. ChIP assay for analysis of STAT3 recruitment into the mouse Mn-SOD promoter after 3 hours of reperfusion with or without IL-6 injection in the cerebral ischemic cortex (A) or in cerebral cortical neurons transfected with IL-6Rα- or gp130-specific siRNA for 24 hours (B). C, Luciferase assay for analysis of transcriptional activity of the Mn-SOD promoter in cerebral cortical neurons transfected with pGLu–Mn-SOD and IL-6Rα- or gp130-specific siRNA or treated with AG490 or IL-6 for 24 hours (n=4 per group). Data are mean± S.E.M. *P<0.05, **P<0.001 vs. control. IR indicates ischemic reperfusion; S, sham; V, vehicle.