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. Author manuscript; available in PMC: 2013 Jan 5.
Published in final edited form as: Nature. 2012 Jul 5;487(7405):57–63. doi: 10.1038/nature11244

Figure 1. The MERVL retrovirus and a reporter driven by its LTR marks the 2C state.

Figure 1

a, Comparison of gene expression between oocytes and 2C embryos. Genes generating junctions to MERVL are shown in red/green, with those in red showing significant change in expression. b, ORF status of predicted MERVL-linked chimeric transcripts. c, GO analysis of MERVL-linked protein coding transcripts. The number of genes from the 10 most enriched GO categories are shown. d-e, 2C (d) and blastocyst embryos (e) were mixed and immunostained with MERVL-Gag and Oct4 antibodies. Scale bar 20 μm. f, Zygotes were injected with the 2C::tomato transgene, and allowed to develop in vitro for 48 hours before imaging. Scale bar 50μm. g, 2C::tomato + ES cells express MERVL-Gag protein detected by immunofluorescence. Scale bar 50μm. h, Microarray analysis of 2C::tomato+ vs. − cells. Red indicates genes with greater than 4-fold change in expression. i, 2C::tomato+/MERVL-Gag+ ES cells and iPS cells lack Oct4 protein as determined by immuno-fluorescence. Scale bar 20μm