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. Author manuscript; available in PMC: 2012 Jul 12.
Published in final edited form as: Hepatology. 2010 Jul;52(1):256–265. doi: 10.1002/hep.23656

Fig. 3.

Fig. 3

Expression levels of oxidative stress-related proteins in the cerebral cortex of control and HE patients. Protein extracts prepared from the cerebral cortex were analyzed for expression levels of nNOS, Hsp27, copper/zinc-dependent superoxide dismutase (CuZnSOD), manganese-dependent superoxide dismutase (MnSOD), and iNOS by western blot analysis. GAPDH served as a loading control. (A) Western blot results taken from three representative control and HE patients. (B) Densitometric quantification of western blot results. Protein expression levels are given relative to GAPDH expression in the individual sample. Each case is shown separately on the vertical scatter plot; bars represent the mean values. *Significantly different compared with the control group. n.s., not significant. (C) Western blot analysis of iNOS expression in the cerebral cortex from control and HE patients. Representative western blot samples (3/8) are shown for both investigational groups. Human hepatoma cells (HepG2) treated with proinflammatory cytokines (tumor necrosis factor-α [10 ng/mL], interferon-γ, interleukin-1β [100 U/mL each, 24 hours]) served as a positive control for iNOS expression.