Table 1. Summary of the FWHM widths measured for the light lines (Fig. 2) and for the PSFs of the system (Fig. 3). An error of one pixel was assumed on the light lines. The mean value and statistical error in the PSFs was obtained over 30 measurements.
| Dimensions of the fluorescent light line |
PSF
from fluorescent beads |
|||
|---|---|---|---|---|
| Modality | Δx (μm) | Δy (μm) | Δr (μm) | Δz (μm) |
| DSLM-Gauss | 173 | 3.8±0.4 | 1.2±0.2 | 2.6±0.5 |
| 2p-DLSM-Gauss | 41 | 3.1±0.4 | 1.5±0.2 | 2.3±0.1 |
| DSLM-Bessel | 278 | 8.9±0.4 | 1.3±0.1 | 10.7±0.9 |
| 2p-DSLM-Bessel | 246 | 2.8±0.4 | 1.4±0.2 | 3.2±0.3 |