Table 1.
Comparisons of YMDD variants in serum of patients with acute and chronic HBV infection detected by direct sequencing and pyrosequencing
| Patient number |
Type of infection |
Treatment |
Duration (months) |
Viral load (cp/mL) |
Sub genotype |
Direct sequencing |
Pyrosequencing % amino acid |
|||
|---|---|---|---|---|---|---|---|---|---|---|
| |
|
|
|
|
|
WT |
MUT |
M |
V |
I |
| ATG | Codon | ATG | (codon) | (codon)/(codon) | ||||||
| 1969 |
acute |
- |
- |
1.1x106 |
A1 |
M/ATG |
- |
100 |
- |
- |
| 2098 |
acute |
- |
- |
1.4x106 |
A1 |
M/ATG |
- |
100 |
- |
- |
| 1377 |
acute |
- |
- |
3.5x104 |
A1 |
M/ATG |
- |
100 |
- |
- |
| 1504 |
acute |
- |
- |
6.2x102 |
A1 |
M/ATG |
- |
100 |
- |
- |
| 1379 |
acute |
- |
- |
2.8x104 |
A1 |
M/ATG |
- |
95 |
- |
5 (ATT) |
| 1419 |
acute |
- |
- |
6.5x103 |
A1 |
M/ATG |
- |
100 |
- |
- |
| 1781 |
acute |
- |
- |
6.6x102 |
A1 |
M/ATG |
- |
95 |
- |
5 (ATT) |
| 1510 |
acute |
- |
- |
8.6x103 |
A1 |
M/ATG |
- |
83 |
- |
17 (ATA) |
| 1384 |
acute |
- |
- |
3.3x105 |
A2 |
M/ATG |
- |
100 |
- |
- |
| 2190 |
acute |
- |
- |
- |
A2 |
M/ATG |
- |
94 |
6 (GTT) |
- |
| 603 |
acute |
- |
- |
1.2x105 |
A2 |
M/ATG |
- |
100 |
- |
- |
| 1472 |
acute |
- |
- |
3.0x103 |
A2 |
M/ATG |
- |
100 |
- |
- |
| 1386 |
acute |
- |
- |
1.3x105 |
A2 |
M/ATG |
- |
96 |
- |
4 (ATT) |
| 1120 |
acute |
- |
- |
7.1x102 |
A2 |
M/ATG |
- |
93 |
- |
7 (ATT) |
| 1393 |
acute |
- |
- |
7.2x103 |
A2 |
M/ATG |
- |
100 |
- |
- |
| 1889 |
acute |
- |
- |
5.6x105 |
A2 |
M/ATG |
- |
96 |
- |
4 (ATT) |
| 1474 |
acute |
- |
- |
5x104 |
D2 |
M/ATG |
- |
100 |
- |
- |
| 1980 |
acute |
- |
- |
2.5x103 |
D2 |
M/ATG |
- |
94 |
6 (GTT) |
- |
| 1314 |
acute |
- |
- |
2.0x104 |
D3 |
M/ATG |
- |
100 |
- |
- |
| 1570 |
acute |
- |
- |
1.3x103 |
F2 |
M/ATG |
- |
94 |
- |
6 (ATT) |
| NN003 |
chronic |
LAM |
01 |
3.7x104 |
A1 |
M/ATG |
|
94 |
- |
6 (ATT) |
| NN004 |
chronic |
LAM |
06 |
1.7 x104 |
A1 |
M/ATG |
|
96 |
- |
4 (ATT) |
| NN124 |
chronic |
LAM |
06 |
9.7 x102 |
A1 |
- |
V/GTG |
- |
40 (GTG) |
60 (ATT) |
| NN092 |
chronic |
LAM |
07 |
7.6 x106 |
A1 |
M/ATG |
- |
100 |
- |
- |
| NN006 |
chronic |
LAM + TDF |
12 |
1.7 x104 |
A1 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN026 |
chronic |
LAM |
12 |
1.2 x107 |
A1 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN041 |
chronic |
LAM |
12 |
1.3 x104 |
A1 |
M/ATG |
- |
94 |
- |
6 (ATT) |
| NN043 |
chronic |
LAM |
12 |
4.2 x105 |
A1 |
M/ATG |
- |
100 |
- |
- |
| NN132 |
chronic |
LAM |
12 |
9.4 x102 |
A1 |
- |
V/GTG |
10 |
75 (GTG) |
15 (ATT) |
| NN123 |
chronic |
LAM |
18 |
2.4 x109 |
A1 |
M/ATG |
- |
94 |
- |
6 (ATA) |
| NN009 |
chronic |
LAM |
24 |
2.1 x104 |
A1 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN024 |
chronic |
LAM |
24 |
5.2 x107 |
A1 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN010 |
chronic |
LAM |
36 |
4.9 x105 |
A1 |
- |
I/ATT |
3 |
- |
93 (ATT)/4 (ATA) |
| NN018 |
chronic |
LAM |
36 |
4.6 x103 |
A1 |
- |
V/GTG |
6 |
94 (GTG) |
- |
| NN019 |
chronic |
LAM |
36 |
3.0 x103 |
A1 |
M/ATG |
- |
96 |
- |
4 (ATA) |
| NN027 |
chronic |
LAM |
36 |
2.8 x104 |
A1 |
M/ATG |
- |
95 |
- |
5 (ATT) |
| NN037 |
chronic |
LAM |
36 |
4.8 x105 |
A1 |
M/ATG |
- |
100 |
- |
- |
| NN079 |
chronic |
LAM |
36 |
9.6 x103 |
A1 |
M/ATG |
- |
100 |
- |
- |
| NN087 |
chronic |
LAM |
72 |
1.1 x104 |
A1 |
M/ATG |
- |
100 |
- |
- |
| NN007 |
chronic |
LAM |
84 |
2.8 x104 |
A1 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN028 |
chronic |
LAM |
108 |
1.8 x109 |
A1 |
V/GTG |
- |
100 (GTG) |
- |
|
| NN032 |
chronic |
LAM + TDF |
132 |
1.2 x104 |
A1 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN025 |
chronic |
LAM |
05 |
4.3 x104 |
A2 |
M/ATG |
- |
100 |
- |
- |
| NN014 |
chronic |
LAM |
07 |
1.4 x105 |
A2 |
- |
I/ATT |
- |
- |
100 (ATT) |
| NN042 |
chronic |
LAM |
12 |
5.4 x107 |
A2 |
- |
V/GTG |
6 |
94 (GTG) |
- |
| NN022 |
chronic |
LAM + ADV |
24 |
1.7 x105 |
B |
- |
I/ATT |
- |
25 (GTG) |
70 (ATT) |
| NN074 |
chronic |
LAM |
06 |
6.5 x105 |
D2 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN125 |
chronic |
LAM + TDF |
12 |
2.5 x103 |
D2 |
- |
I/ATT |
- |
- |
100 (ATT) |
| NN001 |
chronic |
LAM |
60 |
2.4x104 |
D3 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN091 |
chronic |
LAM |
06 |
4.3 x103 |
D3 |
- |
I/ATT |
- |
- |
100 (ATT) |
| NN096 |
chronic |
LAM |
06 |
3.1 x103 |
D3 |
M/ATG |
- |
100 |
- |
- |
| NN097 |
chronic |
LAM |
06 |
5.3 x106 |
D3 |
M/ATG |
- |
95 |
- |
5 (ATT) |
| NN129 |
chronic |
LAM |
06 |
7.2 x108 |
D3 |
- |
V/GTG |
- |
95 (GTG) |
5 (ATT) |
| NN029 |
chronic |
LAM |
12 |
7.0 x104 |
D3 |
M/ATG |
- |
86 |
4 (GTG) |
6 (ATA)/4 (ATT) |
| NN038 |
chronic |
LAM + TDF |
12 |
2.9 x105 |
D3 |
M/ATG |
- |
100 |
- |
- |
| NN077 |
chronic |
LAM |
12 |
9.7 x105 |
D3 |
- |
I/ATT |
4 |
- |
96 (ATT) |
| NN034 |
chronic |
LAM + ADV |
24 |
1.0 x105 |
D3 |
- |
V/GTG |
- |
90 (GTG) |
10 (ATT) |
| NN075 |
chronic |
LAM |
60 |
3.2 x103 |
D3 |
M/ATG |
- |
100 |
- |
- |
| NN031 |
chronic |
LAM |
72 |
6.8 x107 |
D3 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN126 |
chronic |
LAM |
06 |
1.9 x108 |
F1b |
- |
I/ATC |
- |
30 (GTG) |
70 (ATC) |
| NN105 |
chronic |
LAM |
06 |
3.7 x108 |
F2 |
- |
V/GTG |
- |
100 (GTG) |
- |
| NN078 |
chronic |
LAM |
12 |
1.2 x106 |
F2 |
M/ATG |
- |
95 |
- |
5 (ATT) |
| NN134 |
chronic |
LAM |
12 |
2.7 x104 |
F2 |
- |
I/ATT |
- |
25 (GTG) |
75 (ATT) |
| NN020 | chronic | LAM | 48 | 3.7 x104 | F2 | M/ATG | - | 100 | - | - |