Figure 8. Inflammatory, proliferative and immune cells in ilea of deceased “humanized” mice.

“Humanized” (human flora associated) mice were generated as described in Methods, sacrificed by cervical dislocation, and kept at constant ambient conditions. Ileum biopies were taken at defined time points postmortem as indicated on the x-axis. The average numbers of (A) apoptotic cells (positive for caspase-3, Casp3), (B) proliferative cells (positive for Ki67), (C) neutrophilic granulocytes (Neutrophils, positive for MPO7), (D) T-lymphocytes (positive for CD3), (E) regulatory T-cells (positive for FOXP3, Treg), and (F) B-lymphocytes (positive for B220) from at least six high power fields (HPF, x400 magnification) per animal (n = 8) were determined microscopically in immunohistochemically stained ileal paraffin sections. Means (black bars) and levels of significance (P-values) as determined by the Student’s t-test are indicated. Data shown are representative for three independent experiments.