Table 4. List of differentially expressed genes in the gills of Atlantic salmon identified as a potential surrogate of protection against yersiniosis.
| cDNA microarrayProbeAcc. No. | VU vs UU(FC) | Real-time PCRVU vs UU(FC) | VU vs UI72h(FC) | VU vs VI72h(FC) | VU vsUI8h(FC) | VU vsVI8h(FC) | BLAST identification |
| EG929305 | 3.4 ↑ | 1.6 ↑ | 2.5 ↑ | 2.8 ↑ | 2.0 ↑ | 3.2 ↑ | Uncharacterized protein KIAA1033 |
| DY699380 | 3.1 ↑ | 2.4 ↑ | 2.1 ↑ | 1.7 ↑ | 1.8 ↑ | 3.0 ↑ | LIM and actin-binding protein 1 [Salmo salar]* |
| DY729690 | 2.7 ↑ | 3.8 ↑ | 2.2 ↑ | 2.6 ↑ | 2.0 ↑ | 3.2 ↑ | Hepcidin* |
| EG859007 | 2.5 ↑ | 1.5 ↑ | 2.8 ↑ | 2.2 ↑ | 2.3 ↑ | 2.2 ↑ | E3 ubiquitin-protein ligase Itchy |
| CB514941 | 2.4 ↑ | NA | 2.6 ↑ | 1.9 ↑ | 2.9 ↑ | 2.9 ↑ | UDP-N-acetylglucosamine transferase subunit |
| EG811438 | 2.3 ↑ | NA | 1.8 ↑ | 1.5 ↑ | 2.8 ↑ | 2.2 ↑ | Olfactory receptor family C subfamily 4 member 11 |
| DW553700 | 2.1 ↑ | NA | 2.7 ↑ | 1.8 ↑ | 2.1 ↑ | 2.3 ↑ | MAK: Serine/threonine-protein kinase MAK |
| DY723439 | 2.0 ↑ | NA | 2.1 ↑ | 1.5 ↑ | 2.6 ↑ | 1.7 ↑ | Sugar phosphate exchanger 2 [Salmo salar]* |
| DY738009 | 1.9 ↑ | NA | 2.9 ↑ | 1.9 ↑ | 2.6 ↑ | 1.9 ↑ | Alcohol dehydrogenase class-3 |
| CA054083 | 1.8 ↑ | NA | 2.6 ↑ | 2.3 ↑ | 3.1 ↑ | 2.9 ↑ | Immunoglobulin mu heavy chain [O. mykiss]* |
| DY711765 | 1.8 ↑ | NA | 2.7 ↑ | 1.9 ↑ | 2.3 ↑ | 2.0 ↑ | Lamina-associated polypeptide 2 isoforms |
| DW570661 | 1.7 ↑ | NA | 2.0 ↑ | 1.6 ↑ | 2.5 ↑ | 2.0 ↑ | SAM and SH3 domain-containing protein 1 |
| DY712739 | 1.7 ↑ | NA | 2.4 ↑ | 1.7 ↑ | 2.6 ↑ | 2.2 ↑ | AY872256S1 Oncorhynchus mykiss IgH.A |
| DW565729 | 1.6 ↑ | NA | 2.6 ↑ | 1.9 ↑ | 2.0 ↑ | 2.3 ↑ | Fish virus induced TRIM protein [O. mykiss]* |
| CB496376 | 1.7 ↓ | NA | 2.3 ↓ | 1.5 ↓ | 2.9 ↓ | 2.9 ↓ | Myelin and lymphocyte protein |
| EG912256 | 2.0 ↓ | NA | 1.8 ↓ | 1.3 ↓ | 2.6 ↓ | 1.6 ↓ | Bifunctional 3-phosphoadenosine 5-phosphosulfatesynthetase 2* |
| EG779342 | 1.8 ↓ | NA | 1.9 ↓ | 1.4 ↓ | 2.6 ↓ | 1.9 ↓ | Thioredoxin interacting protein [Salmo salar] |
Previously annotated as unknown according to the cGRASP 32 K (Salmonid) cDNA annotation file (Nov. 2008). Blast identification shows the top BLASTX or BLASTN hit with highest total score and lower e-value.
The list of 17 differentially expressed genes (ANOVA P<0.05 and >2.5-fold change) found in common between the comparison of the vaccinated unchallenged group of fish (VU) and all the other treatment/vaccination combinations at both time points post-challenge (UI8h/UI72h and VI8h/VI72h) was considered as a specific vaccine-induced biosignature. Furthermore, this biosignature was considered a potential predictor of vaccine-induced protection after vaccination but before Y. ruckeri challenge and therefore a surrogate of protection. Arrows indicate the direction of the fold change, bold arrows indicate >2.5-fold change, while NA indicates genes not assessed by real-time PCR.