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. 2012 Jul 12;7(7):e40735. doi: 10.1371/journal.pone.0040735

Table 3. Validation of microarray data with quantitative RT-PCR.

Average fold change (Apex/Base) Average fold change (P8/P0)2
Microarray qRT-PCR Pearson’s Correlation Coefficient Microarray qRT-PCR
Group Gene P0(A/B) P8(A/B) P0(A/B) P8(A/B) (P8/P0) (P8/P0)
A1 Stmn2 1.19 1.33 2.38 2.84 0.949 0.21 0.08
Ccnb1 1.37 0.90 1.64 0.89 0.995 0.34 0.24
A2 kcnj10 0.69 0.90 0.42 0.88 0.999 3.71 21.88
Kcnq1 0.82 0.85 0.56 0.52 0.960 2.13 3.17
B Tecta 1.32 5.94 2.20 7.56 0.957 0.22 0.03
Ptprq 0.78 2.11 0.27 2.87 0.837 1.17 1.81
Chrna10 0.84 1.92 0.55 2.96 0.796 1.11 0.83
Fzd4 1.15 1.54 1.74 1.09 0.900 1.39 1.69
Nrcam 1.40 1.99 1.84 2.55 0.989 0.62 0.51
C Tnmd 0.86 0.28 0.81 0.12 0.981 3.95 17.79
Crym 0.98 0.46 1.48 0.24 0.982 0.25 0.12
D Pdzrn4 1.75 1.10 2.80 1.15 0.845 0.84 0.70
Cdh4 1.72 1.31 2.89 1.08 0.997 0.25 0.04
E Fst 3.26 4.25 6.23 13.07 0.982 0.72 0.41
Tectb 2.21 2.22 4.86 12.86 0.953 1.74 2.87
Otog 1.50 2.38 1.48 3.84 0.982 0.71 0.59
Ush1c 1.52 2.12 1.75 3.93 0.967 1.10 0.98
G Atp6v0a4 0.24 0.76 0.06 0.54 0.996 2.92 10.59
Slco1a4 0.42 0.75 0.41 0.36 0.992 8.81 13.70
I Hoxa2 0.32 0.28 0.06 0.01 0.942 1.16 0.85
A2m 0.37 0.40 0.21 0.16 0.997 2.87 3.95
Ppp1r1b 0.50 0.66 0.26 0.35 0.969 2.17 4.95
2

Fold changes between P0 and P8 (P8/P0) were calculated using the average values of the base, middle, and apex for each age.