Table 3. Validation of microarray data with quantitative RT-PCR.
Average fold change (Apex/Base) | Average fold change (P8/P0)2 | |||||||
Microarray | qRT-PCR | Pearson’s Correlation Coefficient | Microarray | qRT-PCR | ||||
Group | Gene | P0(A/B) | P8(A/B) | P0(A/B) | P8(A/B) | (P8/P0) | (P8/P0) | |
A1 | Stmn2 | 1.19 | 1.33 | 2.38 | 2.84 | 0.949 | 0.21 | 0.08 |
Ccnb1 | 1.37 | 0.90 | 1.64 | 0.89 | 0.995 | 0.34 | 0.24 | |
A2 | kcnj10 | 0.69 | 0.90 | 0.42 | 0.88 | 0.999 | 3.71 | 21.88 |
Kcnq1 | 0.82 | 0.85 | 0.56 | 0.52 | 0.960 | 2.13 | 3.17 | |
B | Tecta | 1.32 | 5.94 | 2.20 | 7.56 | 0.957 | 0.22 | 0.03 |
Ptprq | 0.78 | 2.11 | 0.27 | 2.87 | 0.837 | 1.17 | 1.81 | |
Chrna10 | 0.84 | 1.92 | 0.55 | 2.96 | 0.796 | 1.11 | 0.83 | |
Fzd4 | 1.15 | 1.54 | 1.74 | 1.09 | 0.900 | 1.39 | 1.69 | |
Nrcam | 1.40 | 1.99 | 1.84 | 2.55 | 0.989 | 0.62 | 0.51 | |
C | Tnmd | 0.86 | 0.28 | 0.81 | 0.12 | 0.981 | 3.95 | 17.79 |
Crym | 0.98 | 0.46 | 1.48 | 0.24 | 0.982 | 0.25 | 0.12 | |
D | Pdzrn4 | 1.75 | 1.10 | 2.80 | 1.15 | 0.845 | 0.84 | 0.70 |
Cdh4 | 1.72 | 1.31 | 2.89 | 1.08 | 0.997 | 0.25 | 0.04 | |
E | Fst | 3.26 | 4.25 | 6.23 | 13.07 | 0.982 | 0.72 | 0.41 |
Tectb | 2.21 | 2.22 | 4.86 | 12.86 | 0.953 | 1.74 | 2.87 | |
Otog | 1.50 | 2.38 | 1.48 | 3.84 | 0.982 | 0.71 | 0.59 | |
Ush1c | 1.52 | 2.12 | 1.75 | 3.93 | 0.967 | 1.10 | 0.98 | |
G | Atp6v0a4 | 0.24 | 0.76 | 0.06 | 0.54 | 0.996 | 2.92 | 10.59 |
Slco1a4 | 0.42 | 0.75 | 0.41 | 0.36 | 0.992 | 8.81 | 13.70 | |
I | Hoxa2 | 0.32 | 0.28 | 0.06 | 0.01 | 0.942 | 1.16 | 0.85 |
A2m | 0.37 | 0.40 | 0.21 | 0.16 | 0.997 | 2.87 | 3.95 | |
Ppp1r1b | 0.50 | 0.66 | 0.26 | 0.35 | 0.969 | 2.17 | 4.95 |
Fold changes between P0 and P8 (P8/P0) were calculated using the average values of the base, middle, and apex for each age.