Table 4. Functional annotation clustering analysis on the differentially expressed genes.
| Group | Total genes | Enrich. Score1 | Biological annotations | Ratio (%)2 | p-value3 | FDR (%) | Genes |
| A1 | 168 | 8.42 | GO:0022402∼cell cycle process | 13.13 | 1.15E-10 | 1.75E-07 | Bub1 C79407 Ccna2 Ccnb1 Ccnb2 Cdca3 Cks2 Dbc1 Dbf4 Dlgap5 f630043a04rik Hells Kif11 Mki67 Ncaph Plk1 Racgap1 Sgol2 Spag5 Stmn1 Tpx2 |
| 6.86 | GO:0005694∼chromosome | 15.0 | 1.24E-13 | 1.51E-10 | Bub1 C79407 Cenpk F630043a04rik H2afy2 H2afz Hells Hist1h1a Hist1h1b Hist1h2ab Hist1h2af Hist1h2an Hist1h2ao Hist1h2bb Hist1h2bj Hist1h2bl Hist1h2bm Hist1h2bn Hist1h3a Hist1h3b Hist1h3c Hist1h3d Hist1h3e Hist1h3g Hist1h3h Hist1h3i Hist2h3b Hist2h3c1 Hm11277 Hmgb2 Hmgb3 Mki67 Sgol2 Spag5 Tmpo Top2a Zfp57 | ||
| 1.92 | GO:0031012∼extracellular matrix | 6.9 | 3.61E-04 | 0.43868 | Aspn Col6a3 Egfl6 Fbn2 Fras1 Matn1 Oc90 Postn Smoc2 Spock3 Tgfbi | ||
| 1.68 | GO:0022832∼voltage-gated channel activity | 4.4 | 0.00254 | 3.18051 | Cacna1h Kcna6 Kcnh7 Scn2a1 Scn3a Scn3b Scn9a | ||
| 1.51 | GO:0015630∼microtubule cytoskeleton | 10.0 | 7.94E-06 | 0.00968 | Ccnb1 Ckap2 Dlgap5 F630043a04rik Kif2c Kif4 Kif11 Kif15 Kif21b Kif23 Myc Racgap1 Sncg Spag5 Stmn1 Tpx2 | ||
| A2 | 245 | 8.94 | GO:0005578∼proteinaceous extracellular matrix | 9.2 | 4.31E-10 | 5.3E-07 | Abi3bp Chad Chl1 Col4a3 Col4a4 Col8a1 Col24a1 Cpz Ecm2 Emilin2 Entpd2 Fgf1 Gpld1 Kazald1 Kera Lama2 Lgals3bp Npnt Ntn4 Prss36 Spon1 Tnfrsf11b Wnt2b |
| 5.25 | GO:0007155∼cell adhesion | 8.8 | 1.56E-05 | 0.02553 | Anxa9 Atp1b2 Bcam Cd9 Cd36 Cd44 Chl1 Clca5 Cntn4 Col4a3 Col8a1 Col24a1 Emilin2 Itga10 Lama2 Lgals3bp Ninj2 Npnt Sorbs3 Spon1 Tesk2 Wisp1 | ||
| 1.78 | GO:0006811∼ion transport | 8.4 | 0.00113 | 1.82672 | Atp1b2 Atp1b3 Atp2a3 Cacng4 Clca5 Fxyd3 Fxyd5 Glrb Kcne4 Kcnj2 Kcnj10 Kcnj13 Kcnq1 P2rx2 Slc5a5 Slc9a2 Slc10a4 Slc41a2 Slco1c1 Steap3 Tcn2 | ||
| B | 125 | 2.41 | GO:0030182∼neuron differentiation | 0.8 | 0.0015 | 2.28299 | Gata2 Isl2 Lingo1 Mtap2 Nr2e1 Nrcam Slitrk2 Slitrk6 Sox2 Wnt7a |
| 1.88 | GO:0044459∼plasma membrane part | 2.4 | 4.47E-05 | 0.05379 | Apba1 Aqp4 Aqp5 Chrna3 Chrna7 Chrna10 Chrnb4 Cldn9 Cldn23Cubn Cybrd1 Egflam Faim2 Fn1 Kcna10 Kcnb2 Nrcam OtoF Otog Pclo Pla2g4e RgS6 Rgs9 Syt1 Rims2 Six1 Slc6a14 Slc22a3 Sox2 Tecta Tmprss9 Xirp2 | ||
| C | 84 | 2.12 | GO:0005578∼proteinaceous extracellular matrix | 0.9 | 0.00373 | 3.92815 | Acan Crtap Ctgf Nid2 Ntn1 Trf Vcan |
| 1.74 | GO:0006812∼cation transport | 1.2 | 7.95E-04 | 1.13346 | Atp13a5 Kcnh5 Kcnma1 Scn7a Slc4a10 Slc5a3 Slc38a4 Slc39a14 Trf | ||
| D | 46 | 1.87 | GO:0033555∼multicellular organismal response to stress | 7.4 | 7.93E-05 | 0.11043 | P2rx3 Scn9a Tac1 Trpa1 |
| E | 27 | 3.01 | GO:0030182∼neuron differentiation | 28.6 | 9.95E-07 | 0.00138 | Foxg1 Isl1 Jag1 Rorb Robo2 Sema3a Ush1c Ush2a |
| 1.77 | GO:0007423∼sensory organ development | 25.0 | 1.34E-06 | 0.00186 | Foxg1 Jag1 Prox1 Rorb Six2 Ush1c Ush2a | ||
| 1.53 | glycosylation site:N-linked (GlcNAc…) | 46.4 | 0.00117 | 1.44099 | Ndst3 Fst Jag1 Epha3 Pcsk2 Zpld1 Robo2 Chst15 Sema3a Tectb Pla2r1 Muc15 Ush2a | ||
| F | 0 | No gene set was significantly enriched | |||||
| G | 42 | 1.94 | GO:0006811∼ion transport | 21.3 | 7.43E-05 | 0.10516 | Ano5 Atp6v0a4 Atp6v1c2 Cp Gabra5 Kcnj16 Slc17a7 Slco1a4 Tmem38b Wnk4 |
| 1.66 | GO:0007155∼cell adhesion | 17.0 | 5.79E-04 | 0.81684 | Agt Cldn11 Clstn2 Cntn1 Cntn5 Itga4 Pkp1 Vcam1 | ||
| H | 1 | No gene set was significantly enriched | |||||
| I | 5 | No gene set was significantly enriched |
1
Enrichment scores indicate relative importance (enrichment) of the biological annotations.
2
Ratio (%) indicates the percentage of the genes included the enriched annotations among the total number of genes in the group.
3
p-value was calculated with a modified Fisher’s exact test to indicate the significance of the enrichment of the annotations.