Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jul 15;23(14):2658–2670. doi: 10.1091/mbc.E11-12-1056

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Plotnikova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 7: — Mitotic death in cells overexpressing AURKA CaM-interaction-domain mutants. (a) FACS analysis of DNA content for thymidine-arrested HEK293 cells overexpressing RFP-fused AURKA or mutated derivatives or control RFP at 0, 10, or 24 h after release from block. Horizontal brackets indicate 2N and 4N peaks. (b) Quantitative analysis of movie taken at thymidine-blocked cells transfected with AURKA WT, indicated mutants, and vector pcDNA3.1-RFP. Graph shows time of mitotic entry after release from block. *1, p = 0.0004; *2, p = 0.0001; *3, p = 0.00006; *4, p = 0.0001; ns, not significant; compared with RFP control. An average of 100 cells was counted in each of two movies; the box plots represent the 25th percentile, median (50th percentile), and 75th percentile. (c) Analysis of phenotypes indicated from movie described in b. *1, p = 0.004; *2, p = 0.0026; *3, p = 0.0014; *4, p = 0.0052; *5, p = 0.023; * 6, p = 0.0021; *7, p = 0.0016; *8, p = 0.0036; *9, p = 0.0037; *10, p = 0.0016; *11, p = 0.0037; ns, not significant; compared with RFP control. **1, p = 0.0065; **2, p = 0.016; **3, p = 0.026; **4, p = 0.042; **5, p = 0.015; compared with wild-type AURKA. An average of 100 cells was counted in each of two movies; error bars, SE. (d) Representative images from movies statistically analyzed in b and c. Time 0:00 represents initial rounding after release from thymidine. In each case, the rightmost result, at 5 h postrounding, reflects terminal phenotypes observed for cells (i.e., no reattachment was observed in an additional 7 h of observation; many cells floated off plate). For AURKA, the two predominant terminal phenotypes are shown (AURKA, arrest at cytokinesis; AURKA-1, death between metaphase and anaphase).