FIGURE 4:

Overexpression of FLO8 stimulates white-to-opaque switching in 1% CO₂. (A) White-to-opaque switching frequencies in air and in 1% CO₂. The cells were grown on glucose agar with phloxin B at 25°C for 6 d. The WT strain used was WUM5A, an MTLα/α strain derived from the clinically isolated strain WO-1. <, no colonies containing cells of alternative phenotype were observed. (B) Examples of colony and cellular phenotypes in A. Cells collected from a representative plate of each strain were mixed and imaged. Op%, percentage of opaque cells.