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. 2012 Jul 15;23(14):2770–2781. doi: 10.1091/mbc.E12-02-0095

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© 2012 Cesarini et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — ERC formation is stimulated by ncRNA transcription. (A) Schematic map of the rDNA unit. Thin horizontal black arrows, ncRNAs produced from E-PRO and C-PRO promoters. Horizontal white arrow, oligo NTS1-R used in the strand-specific RT-PCR. (B) Quantification of the ncRNA expression level. The histograms indicate the averages and SDs from three independent experiments. Two-tailed t-test was applied for statistical analysis. Asterisks indicate statistically relevant differences; α = 0.05. The p values are as follows: sir2, p = 0.0006; NAM, p = 0.002; rpd3, p = 0.0013; hst1, p = 0.0703; hst2, p = 0.1451; hst3, p = 0.0047; hst4, p = 0.125; nhp6ab, p = 0.0454.