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. 2012 Jul 15;23(14):2793–2804. doi: 10.1091/mbc.E12-02-0103

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© 2012 Iwasawa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 10: — Afadin induces axon branching in an actin-dependent manner. (A) Cortical neurons were transfected with YFP and Myc-tagged afadin FL or ΔC mutant at 1 DIV and fixed at 3 DIV. The fluorescence images of YFP are shown. Scale bar, 50 μm. (B, C) Total axon length (B) and axon tip number per 100 μm (C) were measured. The results are means ± SEM of three independent experiments (n = 45; *p < 0.05, ***p < 0.001; one-way ANOVA with Dunnett's post hoc test). (D, E) Cortical neurons transfected with YFP and Myc-tagged afadin FL at 1 DIV were treated with cytochalasin B (Cyto B; 1 μM), latrunculin A (Lat A; 0.25 μM), or DMSO (vehicle control) for 3 h before fixation at 3 DIV. Total axon length (D) and axon tip number per 100 μm (E) were measured. The results are means ± SEM of three independent experiments (n = 33; ns, not significant; *p < 0.05, ***p < 0.001; one-way ANOVA with Dunnett's post hoc test).