Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jul 15;23(14):2793–2804. doi: 10.1091/mbc.E12-02-0103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Iwasawa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 3: — Knockdown of afadin suppresses axon branching in cultured neurons. (A) Cortical neurons were transfected with YFP and control shRNA or afadin shRNA (#A or #B) before plating using nucleofection technology and lysed at 3 DIV, and the whole-cell lysates were analyzed by immunoblotting. (B) Cortical neurons were transfected with YFP and shRNAs before plating using nucleofection technology and fixed at 3 DIV. The fluorescence images of YFP are shown. Scale bar, 50 μm. (C, D) Total axon length (C) and axon tip number per 100 μm (D) were measured. The results are means ± SEM of three independent experiments (n = 60; ns, not significant; **p < 0.01, one-way ANOVA with Dunnett's post hoc test).