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. 2012 Jul 15;23(14):2793–2804. doi: 10.1091/mbc.E12-02-0103

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© 2012 Iwasawa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Active R-Ras translocates afadin FL, but not afadin ΔRA, to the cell periphery. Neuro2a cells were transiently transfected with GFP (A), GFP + HA-R-Ras QL (B), GFP-afadin FL (C), GFP-afadin ΔRA + HA-R-Ras QL (D), GFP-afadin ΔRA (E), and GFP-afadin ΔRA + HA-R-Ras QL (F). The cells were fixed at 1 d after transfection. The fluorescence images of GFP (A–F, green in merge) and the immunofluorescence images with anti-HA antibody (B, D, F, magenta in merge) are shown. Scale bar, 25 μm.