FIGURE 3:

Deletion of genes that encode SWI/SNF subunits renders cells sensitive to cell wall stress. (A) The indicated strains were spotted on YPD plates without or with 50 μg/ml CR, and plates were incubated for 72 h at 30°C. (B) Zymolyase sensitivity was performed in a 96-well microtiter plate assay with zymolyase 20T, giving concentrations ranging from 125 to 0.12 U/ml YPD. Each well was inoculated with ∼10⁴ cells from an exponentially growing culture. Plates were incubated for 24–48 h at 30°C, and cell growth was determined by measuring absorbance at 550 nm.