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. 2012 Jul 15;23(14):2805–2817. doi: 10.1091/mbc.E12-04-0278

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© 2012 Sanz et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Lack of Swi3 abrogates chromatin remodeling at MLP1 locus. (A) Kinetics of histone H3 binding to MLP1 promoter (MLP1BOX1 region) in WT and swi3Δ strains under CR treatment at the indicated times. (B) Association of histone H3 to MLP1 promoter (MLP1BOX1) in WT, rlm1Δ, slt2Δ, and snf2Δ strains analyzed by ChIP before and after 90 and 180 min of CR addition. In both cases, results are shown as the ratio between the fold enrichment of the treated against the untreated culture. (C) Nucleosome-scanning analysis of MLP1 locus (from −699 to +161) in WT and swi3Δ strains under basal and cell wall stress conditions. Mononucleosomes were isolated from cultures growing in the absence and presence of CR (3 h). Purified DNA samples were analyzed by qPCR as detailed in Materials and Methods. Bottom, nucleosomes represented as ovals at the indicated position of the MLP1 locus.