Figure 8. HPV E6 expression reduces sensitivity to combined ATR inhibition and UVB exposure.

A. Relative viability of HFK cells expressing HPV 5, 8, 38, and 16 E6 was measured following exposure to 10 mJ/cm² UVB in the presence of a gradient of Wortmannin concentrations (µM). Data was normalized to cells exposed to UVB in the absence of Wortmannin for each cell line. n≥3. Error bars represent standard errors of the mean. B. Relative viability of HFK cells expressing HPV 5, 8, 38, and 16 E6 was measured following exposure to 10 mJ/cm² UVB in the presence or absence of Caffeine (2 mM). Data was normalized to cells exposed to UVB in the absence of Wortmannin for each cell line. n≥3. Error bars represent standard errors of the mean. C. Relative viability of HFK cells expressing HPV 5, 8, 38, and 16 E6 was measured following exposure to 10 mJ/cm² UVB in the presence of a gradient of Ku 55933 concentrations (nM). Data was normalized to cells exposed to UVB in the absence of Ku 55933 for each cell line. n≥3. Error bars represent standard errors of the mean. D. Relative viability of HFK cells expressing HPV 5, 8, 38, and 16 E6 was measured following exposure to 10 mJ/cm² UVB with or without Nu 7026 (nM). Data was normalized to cells exposed to UVB in the absence of Nu 7026 for each cell line. n≥3. Error bars represent standard errors of the mean. * denotes a statistically significant difference between cells exposed with and without inhibitor. # denotes a statistically significant difference from LXSN cells for the same concentration of inhibitor.