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. 2012 Jul 12;7(7):e40399. doi: 10.1371/journal.pone.0040399

Figure 5. Cell cycle regulation and TGF-beta signaling in Ezh2-shRNA treated cells.

Figure 5

(A) Real-time quantitative PCR analysis of total RNA extracted from nc-shRNA (control; C) and Ezh2-shRNA treated NSCs and pOLs shows increase in the transcript level of cell cycle arrest genes, however the transcript level of growth differentiation factors (Gdfs) belonging to TGF-beta superfamily increased only in Ezh2-shRNA treated NSCs (consistent with ChIP-seq data). (B) Western blot confirms the upregulation of cell cycle arrest proteins (e.g. p16, a product of Cdkn2a) and the activation of TGF-beta signaling pathway (Smads) in Ezh2-shRNA treated NSCs as compared to the control (C).