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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cancer Discov. 2012 May 3;2(7):624–637. doi: 10.1158/2159-8290.CD-11-0201

Figure 5. P1787-driven Stx2 cytoxicity is selectively activated by the cancer cell environment in vitro.

Figure 5

(a) Representative brightfield microscopy of HeLaCMV-FLuc cells treated with 4 different conditioned, filtered media for 24 hours (+media+P1787; +media+P1787-Stx2; +HeLa+P1787; and +HeLa+P1787-Stx2). Note the dramatic membrane blebbing and apoptotic morphology of +HeLa+P1787-Stx2 conditioned media-treated cells. (b) Bioluminescence imaging of HeLaCMV-FLuc cells treated with increasing amounts of 4 different conditioned, filtered media for 24 hours (bar groups, left to right: 17%, 29%, 44% of total volume per well). *p<0.0005 compared to all other treatments. Inset represents PCR amplification of Stx2A/B mRNA from P1787-Stx2 transformed SB300A1 co-cultured with HeLa cells.