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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: J Med Chem. 2012 Jun 29;55(13):6061–6075. doi: 10.1021/jm300171v

Figure 2.

Figure 2

Validation of α-glucosidases I and II as anti-DENV targets. Establishment of stable cell lines expressing shRNA based on Huh7.5 cells was described previously.12 Indicated stable cell lines expressing small hairpin RNA (shRNA) targeting human α-glucosidase I (sh-glucosidase I), II (sh-glucosidase II) or non-targeting shRNA (control) were infected with DENV at MOI of 0.01 for 2 days. Virus titers in the supernatant were assayed by standard plaque assay. Experiment was performed in triplicate. Values represent average and standard deviation of DENV titer. p values were calculated using student t test (* indicates p < 0.05 compared to control).