Table 1.
Patient No. | CTC/7.5 mL | IE/FACS | Days from draw to IE/FACS | No. of cells collected as input for WGA | aCGH |
---|---|---|---|---|---|
1 | 1 | n.d. | N.A. | N.A. | N.A. |
2 | 1 | n.d. | N.A. | N.A. | N.A. |
3 | 85 | Yes | 1 | 50 | Yes |
4 | 12 | Insufficient sample | N.A. | N.A. | N.A. |
5 | 144 | Failed due to machine error | 1 | N.A. | N.A. |
6 | 6 | Insufficient sample | N.A. | N.A. | N.A. |
7 | 10 | Insufficient sample | N.A. | N.A. | N.A. |
8 | 2 | n.d. | N.A. | N.A. | N.A. |
9 | 590 | Yes | 1 | 20 × 2 | Yes (duplicate) |
10 | 151 | Yes | 4 | 20 | Yes |
11 | 9 | Yes | 2 | 20 | Yes |
12 | 2 | n.d. | N.A. | N.A. | N.A. |
13 | 55 | Yes | 1 | 20 | Yes |
14 | 590 | Yes | 2 | 20 | Yes |
15 | 42 | Yes | 2 | 33 | WGA failed |
16 | 0 | n.d. | N.A. | N.A. | N.A. |
17* | 54 | Yes | 1 | 20 | Yes |
18 | 33 | Yes | 1 | 20 | Yes |
19 | 34 | Yes | 2 | 20 | Hybridization failed |
20* | 12 | Yes | 1 | 18 | Yes |
*Patients with available matched primary tumor
IE/FACS-immunomagnetic enrichment and fluorescence activated cell sorting; aCGH-array comparative genomic hybridization; WGA-whole genome amplification; n.d.-not done; N.A.-not applicable