Table 2.
Correlation analysis of genomic profiles of spiked cells isolated from blood via IE/FACS.
| Cell Line | Source | Input for WGA | No. of replicates | Correlation with positive control | S.D. |
|---|---|---|---|---|---|
| LNCaP | IE/FACS | 20 cells | 2 | 0.63 | 0.15 |
| Cell culture | 50 ng | 1 | 0.82 | n.a. | |
| PC3 | IE/FACS | 20 cells | 2 | 0.88 | 0.03 |
| Cell culture | 50 ng | 1 | 0.97 | n.a. | |
| VCaP | IE/FACS | 20 cells | 2 | 0.82 | 0.02 |
| Cell culture | 50 ng | 1 | 0.89 | n.a. |
Isolated 20 tumor cells (in duplicate) and genomic DNA (50 ng) from cell culture were subjected to WGA and aCGH analysis and compared to positive control: unamplified genomic DNA (600 ng) from cell culture