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. Author manuscript; available in PMC: 2012 Jul 13.
Published in final edited form as: Mol Cell. 2010 Apr 9;38(1):67–77. doi: 10.1016/j.molcel.2010.02.021

Figure 6. Sequence-Dependent Promotion of miR-7 Maturation by SF2/ASF.

Figure 6

(A) Wild-type or mutant miR-7 construct was cotransfected into HeLa cells with a control vector or SF2/ASF-expressing plasmid. Transfection efficiencies were normalized to the EGFP levels (radiolabeled RT-PCR). Precursor and mature miR-7 levels were monitored by northern blotting. The relative activities of SF2/ASF in promoting miR-7 expression are shown in the right panel (t test, p < 0.05; n = 3). Error bars represent SEM. (B) HeLa cells were transfected with either luciferase or SFRS1-specific siRNA. Two days after transfection, cells were harvested to prepare whole-cell extracts, for which the levels of SF2/ASF protein were analyzed by western blotting. In vitro pri-miRNA processing assay was performed with wild-type or mutant pri-miR-7-1 substrates (Michlewski et al., 2008); the results are shown in (C) and (D), respectively.