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. Author manuscript; available in PMC: 2012 Jul 13.
Published in final edited form as: Biol Chem. 2010 Jun;391(6):705–716. doi: 10.1515/BC.2010.066

Figure 2. Effect of Pseudomonas aeruginosa (PAO1)-conditioned medium on the anti- elastase activity of elafin.

Figure 2

Recombinant elafin was incubated with Tryptic Soy Broth (control) or PAO1-conditioned medium (pre-incubated in absence or in presence of the metalloproteinase inhibitors EDTA and phosphoramidon (Pa)) for 0, 1, 2 and 6h and analysed for its inhibitory activity against neutrophil elastase (NE) by spectrophotometry using the NE substrate MeOSuc-AAPV-pNA.