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. Author manuscript; available in PMC: 2012 Jul 13.
Published in final edited form as: Biol Chem. 2010 Jun;391(6):705–716. doi: 10.1515/BC.2010.066

Figure 6. Inactivation of the anti-NE activity of normal BAL fluid by Pseudomonas aeruginosa PAO1-conditioned medium.

Figure 6

Normal BAL fluid was incubated with Tryptic Soy Broth (control) or PAO1-conditioned medium, pre-incubated in absence or presence of EDTA and phosphoramidon (Pa) for 0, 1, 2 and 6h and analysed for its inhibitory activity against neutrophil elastase (NE) by spectrophotometry using the NE substrate MeOSuc-AAPV-pNA.