Skip to main content
. 2011 Nov 22;21(11):1936–1947. doi: 10.1089/scd.2011.0422

FIG. 1.

FIG. 1.

DLX3 overexpression or DLX3 silencing in dental follicle stem cells (DFCs). The gene expression of DLX3 was determined by quantitative reverse- transcription polymerase chain reaction (qRT-PCR) analysis after 48 h of transfection with the expression plasmid (pDLX3) and the empty vector (pEV) (A) or with two independently DLX3-specific small interfering RNA (siRNA), siRNA(6) and siRNA(7), and a nonspecific siRNA, NS siRNA (C). All values are means plus standard error (σ/√n) of three biological replicates (n=3) per group. Gene expression of DFCs after transfection with pDLX3 or DLX3 siRNA was compared with the control groups pEV or NS siRNA, respectively. DLX3 expression were also verified at protein level by western blotting (B,D); recombinant DLX3 was detected with an epitop-V5 specific antibody and the total DLX3 protein was detected with a specific antibody DLX3. The β-Actin antibody was used as a housekeeper standard. Protein lysates after 72 h of transfection (for details, see Materials and Methods) were used for western blot analysis.