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. 2011 Nov 22;21(11):1936–1947. doi: 10.1089/scd.2011.0422

FIG. 6.

FIG. 6.

Influence of DLX3 on osteogenic differentiation of DFCs. The differentiation was evaluated by measurement of the ALP activity (A, B). ALP activity was quantified in DFCs 10 days after induction with osteogenic differentiation medium (ODM) and transfection with (A) pDLX3 and pEV or (B) DLX3 specific siRNAs (6 and 7). ALP activities were calibrated to the activity of the respective control cultures, pEV or NS siRNA, with ODM. All values are means plus standard error (σ/√n) of 4 biological replicates per group. Significant differences are shown with the Student's t test (n=4; *P<0.05). Relative gene expression of ALP was determined 3 days after induction with ODM and transfection with (C) pDLX3 and pEV or a (D) DLX3 specific siRNA(6). Mineral deposits in DFC cultures were estimated with alizarin red staining after 28 days of culture with ODM and transfection with (E) pDLX3 and pEV or (F) DLX3 siRNA(6) and NS siRNA. Quantification of alizarin staining was normalized to the respective controls, pEV or NS siRNA, cultivated in ODM. All values are means plus standard error (σ/√n) of 3 biological replicates per group. Significant differences are shown with the Student's t test (**: p<0.005).