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. Author manuscript; available in PMC: 2012 Jul 13.
Published in final edited form as: Development. 2007 Nov;134(22):4083–4093. doi: 10.1242/dev.008524

Fig. 7. Reduced proliferation and early progenitor cell cycle exit in cD2−/− MGE.

Fig. 7

All sections, 4 µm. (A) Fewer nuclei label with the M-phase marker PH3 in the VZ and SVZ of the cD2−/− MGE than in WT. (B–D) Fewer nuclei are labeled by a 1-hour BrdU pulse in cD2−/− (C) than WT (D) MGE. The proportion of total (DAPI) nuclei labeled by anti-BrdU is significantly reduced in the SVZ. (E–G) Dual-immunolabeling with BrdU and Ki67 (to label S-phase through M-phase cells) shows the total numbers of nuclei labeled by a 24-hour BrdU pulse is slightly reduced in cD2−/− MGE, but numbers of BrdU+ nuclei not co-labeled with Ki67+ are increased in the MGE of cD2 nulls (F), as compared with WT (E). Quantification of dual (P fraction) and single (Q fraction; BrdU alone) labeled cells indicates a decrease in the proliferating, P fraction and a corresponding increase in the quiescent, Q fraction in cD2−/− MGE (G), suggesting progenitors are prematurely depleted in cD2−/− mice. *, P<0.05; **, P<0.01; ***, P<0.003.