Table 2.
Haemodynamic changes
| Sham | Saline-fresh | Saline-stored | LPS-control | LPS-fresh | LPS-stored | |
|---|---|---|---|---|---|---|
| (n = 5) | (n = 4) | (n = 3) | (n = 6) | (n = 5) | (n = 5) | |
| PAP (mmHg) | 14.7 ± 0.7 | 18.4 ± 0.7 | 24.7 ± 0.7 | 27.0 ± 0.6 | 30.7 ± 0.7 | 37.0 ± 0.7a, b, c |
| (10 to 19) | (4 to 28) | (9 to 60) | (5 to 47) | (6 to 62) | (13 to 89) | |
| MAP (mmHg) | 108.3 ± 2.2 | 107.3 ± 2.3 | 110.6 ± 2.3 | 90.0 ± 2.0 | 89.0 ± 2.3 | 98.1 ± 2.3a, c |
| (67 to 146) | (64 to 172) | (55 to 155) | (37 to 130) | (44 to 169) | (30 to 149) | |
| CVP (mmHg) | 6.0 ± 0.6 | 5.1 ± 0.6 | 5.5 ± 0.6 | 5.7 ± 0.6 | 4.8 ± 0.6 | 5.2 ± 0.6a |
| (1 to 20) | (0 to 13) | (0 to 20) | (0 to 17) | (0 to 18) | (1 to 18) | |
| Heart rate (bpm) | 102.5 ± 2.9 | 112.9 ± 3.0 | 113.7 ± 3.0 | 111.4 ± 2.7 | 121.8 ± 3.0 | 122.6 ± 3.0a |
| (65 to 147) | (60 to 132) | (70 to 192) | (52 to 210) | (57 to 182) | (65 to 166) | |
| O2sat (%) | 97.2 ± 0.6 | 97.7 ± 0.7 | 96.8 ± 0.7 | 94.9 ± 0.6 | 95.4 ± 0.7 | 94.5 ± 0.7a |
| (81 to 100) | (80 to 100) | (86 to 100) | (69 to 100) | (73 to 100) | (71 to 100) | |
| EtCO2 (mmHg) | 32.9 ± 1.2 | 32.5 ± 1.2 | 30.6 ± 1.2 | 34.0 ± 1.2 | 33.6 ± 1.2 | 31.7 ± 1.2 |
| (20 to 47) | (23 to 39) | (10 to 44) | (13 to 54) | (17 to 52) | (15 to 55) | |
| CO (L/min) | 4.6 ± 0.2 | 4.6 ± 0.2 | 3.7 ± 0.2 | 4.5 ± 0.2 | 4.5 ± 0.2 | 3.6 ± 0.2a, b, c |
| (3.1 to 6.6) | (3.5 to 6.0) | (1.8 to 7.2) | (1.1 to 7.1) | (1.1 to 5.8) | (1.4 to 5.0) | |
| SvO2 (%) | 70.2 ± 1.5 | 73.3 ± 1.5 | 65.9 ± 1.5 | 72.5 ± 1.4 | 75.6 ± 1.5 | 68.3 ± 1.5a |
| (44 to 82) | (33 to 81) | (38 to 84) | (48 to 91) | (48 to 86) | (22 to 77) | |
| Body temperature (°C) | 38.7 ± 0.1 | 38.5 ± 0.1 | 38.7 ± 0.1 | 38.7 ± 0.1 | 38.5 ± 0.1 | 38.7 ± 0.1 |
| (38.1 to 39.8) | (37.2 to 40.2) | (38.0 to 39.4) | (38.0 to 41.4) | (37.1 to 41.5) | (37.7 to 41.1) | |
| PaO2 (mmHg) | 184.8 ± 11.9 | 193.2 ± 13.0 | 152.6 ± 13.1 | 131.1 ± 7.4 | 139.5 ± 13.2 | 98.9 ± 13.2a, b, c |
| (122 to 216) | (76.5 to 210) | (72.2 to 205) | (94.8 to 215) | (64.8 to 233) | (50.0 to 210) | |
| PaCO2 (mmHg) | 37.7 ± 2.0 | 37.0 ± 2.2 | 39.3 ± 2.2 | 39.8 ± 1.4 | 39.1 ± 2.1 | 41.4 ± 2.2a |
| (30.5 to 42.3) | (24.9 to 40.6) | (22.7 to 51.5) | (28.5 to 54.5) | (23.7 to 51.9) | (27.7 to 70.6) | |
| Cstat (L/kPa) | 38.4 ± 3.7 | 35.1 ± 4.0 | 30.4 ± 4.0 | 27.4 ± 2.5 | 24.1 ± 4.0 | 19.4 ± 4.0a, b, c |
| (26.3 to 63.6) | (22.3 to 39.0) | (15.9 to 43.6) | (14.2 to 73.3) | (12.0 to 65.0) | (9.3 to 66.7) | |
| PaO2/FiO2 (FiO2 = 40%) | 462.0 ± 11.9 | 483.0 ± 13.0 | 381.5 ± 13.1 | 327.8 ± 7.4 | 348.8 ± 13.2 | 247.3 ± 13.2a, b, c |
| (305 to 540) | (191 to 525) | (181 to 513) | (237 to 537) | (162 to 583) | (125 to 525) |
CO, cardiac output; Cstat, static pulmonary compliance; CVP, central venous pressure; EtCO2, end tidal carbon dioxide; FiO2, fraction of inspired oxygen; "fresh PRBC", pooled heat-inactivated supernatant from day 1 human packed red blood cell concentrates; LPS, lipopolysaccharide; LPS-control, group of sheep that received LPS followed by saline; LPS-fresh, group of sheep that received LPS followed by "fresh PRBC"; LPS-stored, group of sheep that received LPS followed by "stored PRBC"; MAP, mean arterial pressure; O2sat, oxygen saturation; PaCO2, arterial partial pressure of carbon dioxide; PaO2, arterial partial pressure of oxygen; saline-fresh, group of sheep that received saline followed by "fresh PRBC"; saline-stored, group of sheep that received saline followed by "stored PRBC"; PAP, pulmonary artery pressure; SEM, standard error of the mean; sham, group of sheep that received saline followed by saline; "stored PRBC", pooled heat-inactivated supernatant from day 42 human packed red blood cell concentrates; SvO2, mixed venous oxygen saturation; TRALI, transfusion-related acute lung injury.
Averages ± SEM (minimum-maximum) for each group are shown. Averages and SEM were calculated using mixed models. Minimum and maximum values were from recorded data. a P < 0.05 vs. sham. b P < 0.05 vs. LPS -control. c P < 0.05 vs. LPS-fresh.