ChIP assay buffers
1× RIPA Buffer (50 ml)
Final concentration:	Volume of stock buffers:
10 mM Tris–HCl, pH 7.5	500 μl of 1 M
1 mM EDTA	100 μl of 0.5 M
1% Triton X-100	5 ml of 10%
0.1% SDS	500 μl of 10%
0.1% Nadeoxycholate	500 μl of 10%
100 mM NaCl	1 ml of 5 M
Water	42.4 ml
2× RIPA Buffer (50 ml)
20 mM Tris–HCl, pH 7.5	1 ml of 1 M
2 mM EDTA	200 μl of 0.5 M
2% Triton X-100	10 ml of 10%
0.1% SDS	500 μl of 10%
0.2% Nadeoxycholate	1 ml of 10%
200 mM NaCl	2 ml of 5 M
Water	35.3 ml
Lysis Buffer (10 ml)
50 mM Tris–HCl, pH 8.0	500 μl of 1 M
10 mM EDTA	200 μl of 0.5 M
1% SDS	1 ml of 10%
Water	8.3 ml
TE Buffer (50 ml)
10 mM Tris–HCl, pH 8.0	500 μl of 1 M
10 mM EDTA	1 ml of 0.5 M
Water	48.5 ml
Elution Buffer (50 ml)
20 mM Tris–HCl, pH 7.5	1 ml of 1 M
5 mM EDTA	500 μl of 0.5 M
50 mM NaCl	500 μl of 5 M
Water	48 ml

Containing 1% SDS and 50 μg/ml proteinase K prior to use

All buffers must be 0.2 uM filtered sterilized, and are based on [41]