Fig. 3.

GFP expression at the RNA level in PCC6803. The Synechocystis sp. PCC 6803 cells of the wild type (WT) or transconjugants harboring pGFP500 (+AU) or pGFP461c (−AU) were grown under continuous white light (35 μE m^-2 s⁻¹) in the CB medium for 12 days, then exposed to a 12-h light (L)/12-h dark (D)/12-h light (L) cycle. The cells were harvested at specific times [3 h (L), 15 h (D), and 27 h (L)]. Total RNA (10 μg) was prepared from the cells and subjected to primer extension analysis with the ³²P-end-labeled specific primer, gloGFP-R. Relative signal intensities (+1 as the transcription start point) on an X-ray film are presented for transcripts, with a 100 % value referring to that after 3 h of light in the GFP461c strain. Relative average values (in percent) are shown as bars with error values (standard deviation, SD) obtained from independent triplicate experiments