Fig. 8.

PCR analysis of recombinant S. elongatus PCC 7942 (a) and Lymnothrix/Pseudanabaena sp. ABRG5-3 (b) containing GFP expression vectors. Per strain, 1 μg of total DNA was analyzed by the same procedure as described in Fig. 2a. c Southern blot analysis of each expression vector’s copy number in PCC 7942 and ABRG5-3 transconjugants. The DNA of pVZ321 (Vec), pAM500 (+AU), and pAM461c (−AU) (left block, 0.0133 pmol) digested with the restriction enzymes HindIII and XhoI was subjected to hybridization with a specific probe as described in Fig. 2b for the concentration marker which was used for the trial calculation of plasmid copy numbers in the cells (Table 2). Total DNA (20 μg) was isolated from the respective transconjugants of PCC 6803, PCC 7942, and ABRG5-3, digested by the restriction enzymes, and also subjected to hybridization with the specific probe. Of note is that the exposure time for the X-ray film was approximately 1.5 times longer than that of Fig. 2b