Table 1.
Bacterial strains and plasmids used in this study
| Strains or plasmids | Genotype, phenotype and other characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | F-endA1 supE44 λ-thi-1 recA1 gyrA96 | Cosmo Bio |
| DH5αMCR | deoRΔ(lacZYA-argF)U169 | (Tokyo, Japan) |
| Escherichia coli | E. coli B F − ompT hsdS (r−B m−B) dcm + | Stratagene |
| BL21-CodonPlus | gal λ (DE3) endA The [argU ileY leuW CmR], TetR | (North Torrey Pines RoadLa Jolla, USA) |
| (DE3)-RIL | ||
| Synechocystis sp. | Wild type (WT) | Kazusa DNA |
| PCC 6803 | Research Institute | |
| 6803_pVZ321a | PCC6803 transconjugant with pVZ321 (CmR, KmR) | This study |
| 6803_GFP500 | PCC6803 transconjugant with pGFP500 (CmR) | This study |
| 6803_GFP461c | PCC6803 transconjugant with pGFP461c (CmR) | This study |
| Synechococcus elongatus | Wild type (WT) | Univ. of Tokyo |
| PCC 7942 | ||
| 7942_pVZ321 | PCC7942 transconjugant with pVZ321 (CmR, KmR) | This study |
| 7942_GFP500 | PCC7942 transconjugant with pGFP500 (CmR) | This study |
| 7942_GFP461c | PCC7942 transconjugant with pGFP461c (CmR) | This study |
| Limnothrix/Pseudanabaena sp. | Wild type (WT) | Nishizawa et al. (2010) |
| ABRG5-3L1 | ||
| 5-3_pVZ321 | ABRG5-3 transconjugant with pVZ321 (CmR, KmR) | This study |
| 5-3_GFP500 | ABRG5-3 transconjugant with pGFP500 (CmR) | This study |
| 5-3_GFP461c | ABRG5-3 transconjugant with pGFP461c (CmR) | This study |
| Plasmids | ||
| pAG500 | pAM990 + M. aeruginosa K-81 psbA2 a | Agrawal et al. (1999) |
| -404/+113 (SmaI–BglII fragment, WT), ApR SpR | ||
| pAG461 | pAM990 + M. aeruginosa K-81 psbA2 | Agrawal et al. (2001) |
| −404/+113 (SmaI–BglII fragment, ΔAT), ApR SpR | ||
| pAD461c | pAM990 + M. aeruginosa K-81 psbA2 | This study |
| -404/+113 (SmaI–BglII fragment, ΔAT and a deletion of G at the position of +36), ApR SpR | ||
| pGLO | E. coli expression vector for gfp, ApR | Clontech (Mountain View, USA) |
| R751 | IncP Tra+, TpR | Meyer and Shapiro (1980) |
| pVZ321 | IncQ Mob+, KmR CmR | Zinchenko et al. (1999) |
| pAM500 | Cyanobacterial expression vector (pVZ321 + PpsbA2_WT), CmR | This study |
| pAM461c | Cyanobacterial expression vector (pVZ321 + PpsbA2_ΔAT), CmR | This study |
| pGFP500 | pAM500 + gfp derived from pGLO, CmR | This study |
| pGFP461c | pAM461c + gfp derived from pGLO, CmR | This study |