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(A) COS7 cells exposed to 30 seconds of insonation in presence of pDNA-loaded nanobubbles carrying 10 μg/mL of pDNA and examined 24 hours post transfection by confocal laser scanning microscopy without fixation. (B) COS7 cells treated as in (A) but not sonified. (C) COS7 cells neither exposed to ultrasound nor to DNA-loaded nanobubbles.
Note: The upper panels show fluorescence images while the lower panels show merged phase-contrast and fluorescence images.
