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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Hepatology. 2012 Jul 12;56(3):1140–1149. doi: 10.1002/hep.25736

Figure 4. Cyclin E1 is essential for CCl4-mediated liver fibrosis.

Figure 4

CcnE1−/− knockout mice and WT controls were periodically (twice a week) treated with CCl4 for 4 weeks and sacrificed 48 h after the last injection. (A) Sirius red staining of liver paraffin sections after 4 weeks of CCl4-treatment. Collagen fibres are stained in red. (B) Quantification of Sirius red stained tissue area using image analysis software. (C) Quantification of COL1A1 mRNA expression in CCl4-treated liver samples by quantitative real-time PCR. (D) Quantification of α-SMA mRNA expression. (E) Protein expression of α-SMA and PCNA. Liver extracts from oil-treated WT- and CcnE1−/− mice were used as controls. **: p<0.01; ***: p< 0.001.