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. 2012 Jun 25;109(28):11200–11205. doi: 10.1073/pnas.1207290109

Fig. 1.

Fig. 1.

Bone marrow-derived macrophages from p38γ/δ-deficient mice show altered cytokine production in response to LPS. (A) BMDM from WT (black bars) or p38γ/δ−/− (white) mice were exposed to LPS (100 ng/mL) for the indicated times, and culture supernatants harvested for luminex cytokine analysis of TNFα, IL-1β, IL-10, IL-6, and IL-12(p70). Values show mean ± SD for one representative experiment of three performed in duplicate. (B) qPCR of TNFα, IL-1β, IL-10, IL-6, IL-12(p35), and IL-12(p40) mRNA in total RNA from WT (black bars) or p38γ/δ−/− (white) BMDM stimulated with LPS (100 ng/mL). Results were normalized to 18S RNA expression and x-fold induction was calculated relative to WT expression at 0 h. Data show mean ± SD from one representative experiment of two in triplicate, with similar results. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 relative to WT BMDM exposed to LPS at each time. (C) WT and p38γ/δ−/− BMDM were exposed to LPS (100 ng/mL). Cell lysates (50 μg) were immunoblotted with a murine anti-TNFα antibody.