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. 2012 Jun 25;109(28):11200–11205. doi: 10.1073/pnas.1207290109

Fig. 2.

Fig. 2.

Role of IL-10 in LPS-induced cytokine production. (A and B) BMDM from WT (black bars) or p38γ/δ−/− (white) mice were exposed to LPS (100 ng/mL) alone or in the presence of neutralizing antibody to IL-10 (anti-IL10) or an isotype control antibody (IgG) (both at 1 μg/mL, 12 h); culture supernatants were harvested for luminex cytokine analysis of (A) IL-12(p70) and IL-12(p40) and (B) IFNβ. Data are mean ± SD of one representative experiment of two in triplicate with similar results. Real-time qPCR analysis of (A) IL-12(p35), IL-12(p40), and (B) IFNβ in total RNA from WT (black bars) or p38γ/δ−/− (white) BMDM stimulated as before for the times indicated. Results show mean ± SD of triplicate wells normalized to GAPDH mRNA. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 relative to WT BMDM in each experimental condition.