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. 2012 Jun 25;109(28):11200–11205. doi: 10.1073/pnas.1207290109

Fig. 3.

Fig. 3.

p38γ and p38δ are necessary for LPS activation of ERK1/2 but not of other signaling pathways. (A) Scheme of various signaling pathways activated in macrophages after LPS stimulation. (B–D) BMDM from WT, p38γ−/−, p38δ−/−, or p38γ/δ−/− mice were stimulated with 100 ng/mL LPS for the times indicated (B and C) or for 15 min (D). Cell lysates (50 μg) were immunoblotted with antibodies to (B) active phosphorylated p38α (P-p38α) and active phosphorylated JNK1/2 (P-JNK1/2) or IκBα and (C) active phosphorylated ERK1/2 (P-ERK1/2) and MKK1 (P-MKK1). (B and C) Total protein levels of tubulin, p38MAPK, JNK1/2, ERK1/2, and MKK1 were also measured in the same lysates as loading controls. Duplicate lanes are shown; similar results were obtained in at least three independent experiments. (D) Cell lysates immunoblotted with anti–TPL-2, anti–ABIN-2, anti-phospho p105 (P-p105), anti-p105, anti–P-ERK1/2, anti–ERK1/2, anti–P-MKK1, or anti–MKK1.